11. Detrimental Effect of Lithium Carbonate on Cerebellar Purkinje Neurons in Albino Rats
1. Tazeen Kohari 2. Naheed Najmi
1. Asstt. Prof. of Anatomy, 2. Assoc. Prof. of Operative Dentistry, Liaquat College of Medicine and Dentistry, Karachi
ABSTRACT
Objective: To observe the damaging effect of Lithium carbonate on Purkinje neuron in the light of available literature so this study was under taken to see the toxic effects of Lithium carbonate on permanent Purkinje neuron. Study Design: Experimental study
Place and Duration of study: This study was carried out at Animal House, BMSI, JPMC, Karachi from April 2012 to June 2012.
Materials and Methods: Thirty male albino rats of 190-240 grams were selected and divided into two major groups (control) A and B (Lithium Carbonate-treated) comprising of 15 animals each. According to the time period of the study which was 2 weeks, 6 weeks and 12 weeks. Group A served as control group which was given normal diet and B was given Li2CO3 in powder form mixed in flour. Lithium carbonate was given at a dose of 20mg/kg/day for two, six and twelve weeks. At the end of 2nd, 6th and 12th weeks the cerebellum was removed and fixed in 10%
formal saline. After processing in paraffin five micron thick section were pre ared four Purkinje cell count. Results: Group A revealed normal Purkinje cell count but the mean values f the Purkinje cell count in group B was highly significantly decreased p-value<0.001 as compared to group A.
Conclusion: Our study concludes that acute and chronic ingestion of lithium carbonate at therapeutic level is detrimental to the survival of Purkinje cerebellar neuron and patients who are prescribed lithium carbonate should be monitored carefully.
Key Words: cerebellum, Purkinje, neuron, Lithium carbonate.
Citation of article: Kohari T, Najmi N. Detrime tal Effect of Lithium Carbonate on Cerebellar Purkinje Neurons in Albino Rats. Med Forum 2016;27(2):41-44.