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  3. 13.Compare Oxidative Role of Beta-Carotene and Resveratrol (3 4 5 Hydroxystillbene) in Methotraxate Induced Hepatotoxicity on the Basis of Morphology and Catalase Activity
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13.Compare Oxidative Role of Beta-Carotene and Resveratrol (3 4 5 Hydroxystillbene) in Methotraxate Induced Hepatotoxicity on the Basis of Morphology and Catalase Activity

Syed Muhammad Masood Ali1, Sara Sughra3, Sonia Khan1, Iqbal Ahsan4, Sayyada Humaira Masood2 and Fareeda Islam4

ABSTRACT

Objective: Compare the oxidative role of β-carotene (BC) and Resveratrol (RSV) in Methotrexate (MTX) induced liver toxicity based on morphology and catalase activity.

Study Design: Experimental longitudinal study

Place and Duration of Study: This study was conducted at the Al-Tibri Medical College & Hospital from January 2021 to October 2021 for a period of 10 months.

Materials and Methods: 48 healthy Wistar albino rats were attained and then divided into 6 groups, Group I (Control Group) received normal saline, Group II was given BC, Group III was given RSV, Group IV was given MTX, Group V was given BC+MTX, and Group VI was given RSV+MTX for 24 days. On the 25th day the subjects were euthanized and liver sections for studying the nuclear diameter was fixed with formalin and stained using H&E staining. The nuclear diameter was measured with ocular counting scale on a light microscope.  The liver homogenate was used to analyze catalase enzyme content. Data was analyzed using SPSS with all variables given in Mean± S.D. for statistical analysis paired-t test was done with the level of significance being P-value <0.05.

Results: Mean Nuclear Diameter of Hepatocytes were 5.067 ± 0.1256 in G-I, 5.233±0.0422 in G-II, 5.217±0.0601 in G-III, 5.733±0.0803 in G-IV, and 5.483±0.0307 in G-V, and 5.467±0.0494 in G-VI.  Significant difference was seen when all the groups were compared with the MTX Group (G-I vs G-IV P=0.008, G-II vs G-IV P=0.002, G-III vs G-IV P=0.006, G-V vs G-IV P=0.048, & G-VI vs G-IV P=0.042).  The level of CAT in each Group in (U/mg protein) were 143.5±3.21 in G-I, 143.15±2.87 in G-II, 149.17±3.85 in G-III, 92.71±1.93 in G-IV, 123.09±3.19 in G-V, and 125.5±1.95 in Group VI. Significant difference was seen when all the groups were compared with the MTX groups (G-I VS G-IV P=0.0001, G-II VS G-IV P=0.001, G-III VS G-IV P=0.001, G-V VS G-IV P=0.01, & G-VI VS G-IV P=0.01).

Conclusion: According to the study results both therapeutic agents were significantly effective in prevention of the toxic effects of methotrexate on liver. There potent antioxidant effect was evidenced through morphological restoration of liver architecture and catalase activity.

Key Words: methotrexate, liver, catalase

Citation of article: Ali SMM, Sughra S, Khan S, Ahsan I, Masood SH, Islam F. Compare Oxidative Role of Beta-Carotene and Resveratrol (3 4 5 Hydroxystillbene) in Methotraxate Induced Hepatotoxicity on the Basis of Morphology and Catalase Activity. Med Forum 2022;33(1):52-56.