14. Pentoxifylline Protects against Carbon Tetrachloride Induced Liver Injury in Adult Male Wistar Rat Model
1. Maria Kazi 2. Muhammad Bachal Pandhiani 3. Salman Ahmad Farsi Kazi
4. Saif-ur-Rehman 5. Haji Kjan Khoharo
1. Asstt. Prof. of Biochemistry, Al-Tibri Medical College, Karachi 2. Asstt. Prof. of Anatomy, Al-Tibri Medical
College, Karachi 3. Asstt. Prof. of Forensic Medicine, Faculty of Medicine and Allied Medical Sciences, Isra
University, Hyderabad 4. Asstt. Prof. of Physiology, Faculty of Medicine and Allied Medical Sciences, Isra
University, Hyderabad 5. Asstt. Prof./Consultant Physician, Dept. of Physiology/Medicine, Faculty of Medicine and Allied Medical Sciences, Isra University, Hyderabad
ABSTRACT
Objective: To investigate the protective effect of Pentoxifylline (PTX) in carbon tetrachloride (CCl4) induced liver injury in adult male Wistar rat model.
Study design: Experimental/Analytical study
Place and Duration: Animal House, Isra University Hyderabad from May to December 2012.
Subjects and Methods: Forty five adult male Wistar rats were divided into three groups; Group A. controls received 0.9% isotonic saline, Group B. received CCl4 orally (1.9mg/kg) mixed in olive oil, and Group C. received the PTX+ CCl4. Blood samples were collected for liver biochemical assays. The animals were sacrificed, liver tissue, after fixation in 4% formaldehyde, was embedded in paraffin. Tissue sections of 5μ thickness were subjected to haematoxylin and eosin staining and were assessed by light microscopy. The data was analyzed on SPSS 21.0 using one-way ANOVA, Tukey-Cramer and Chi-square tests. A p-value of ≤ 0.05 was taken statistically significant. Results: The liver biochemical and histological findings reveal statistically significant differences among the controls, CCl4 and PTX+ CCl4 groups (p=0.0001). Liver enzymes and histology was deranged significantly in CCl4 group compared to controls and PTX+ CCl4 group (p=0.0001). The CCl4+PTX group shows less elevation of liver enzymes and derangement in liver histology when compared to CCl4 group (p=0.001). The histological findings of congestion, inflammatory cell infiltrate, vacuolar degeneration and necrosis are found prominent in CCl4 group. Conclusion: The Pentoxifylline protects against oxidative damages caused by carbon tetrachloride induced liver injury in rat model.
Key words: Carbon tetrachloride, Liver injury, Pentoxifylline